Charlton will be ball number 10 in Friday's Bristol Street Motors Trophy round of 32 draw.
The draw is set to take place from 6.30pm GMT and will be shown live on Sky Sports News.
Having progressed as runners-up in Southern Group B, the Addicks will face either Portsmouth, MK Dons, Reading or West Ham United U21s in the round of 32.
Unless Charlton draw the young Hammers, the tie will take place away from home.
Round of 32 ties are scheduled to be played in the week commencing December 4th.
Bristol Street Motors Trophy - Southern Section Draw B
Winners pot | Ball no. | Runners-up pot | Ball no. |
Portsmouth | 5 | Brighton U21s | 9 |
MK Dons | 6 | Charlton | 10 |
Reading | 7 | AFC Wimbledon | 11 |
West Ham United U21s | 8 | Colchester United | 12 |